Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The SpectraMax® iD3 Multi Mode Microplate . The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. View the Molecular Devices website. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Setup recommendations for SpectraMax. Assay setup for a 384-well low-volume plate. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Product Keyword. 5mM), and changes in OD 405 nm. StakMax. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. Acceptor: 665nm. SoftMax Pro. 4. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. 001/min at 425 nm. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). Acceptor filter: 665 nm. 3 mm. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Emission. SpectraMax Multi-Mode Microplate Readers. Buffer (100 mmol L –1acetate buffer, pH 5. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. When necessary, samples were. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Details. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Softmax PRO v5. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Molecular Devices SpectraMax M5e Microplate Reader. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Acceptor: 665/10 nm. The absorbances were being measured at 515 nm. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Lactate Dehydrogenase (LDH) Release. 2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The initial rate of hydrolysis was determined using the early linear region of. 30. Software (SoftMax Pro ® software or comparable plate reader software). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Amino Acid Analysis. , Oxon, UK). ScanLater Western Blot Detection Cartridge. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. SpectraMax Gemini EM & FLEXstation (bottom read) 1. Triton X-100 (see Note 2). On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. #N207) LAL Reagent Water (Lonza cat. Example: [Drawer]. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. 4. Details. 2. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. We also offer depot Repair Service from our base of operations in Southern California (San Diego). 6. 5. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Number of flashes. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. Intra- and inter-assay coefficients of variation were lower than 8. SpectraMax Multi-Mode Microplate Readers. 2 Chemical Reagents 1. 94 g calcium chloride dihydrate (both. Molecular Devices Spectramax 340PC instructions. FACS analysis. The area under the curve (AUC) for the comparison of the growth rates was determined using. MCE has not independently confirmed the accuracy of these methods. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 0 3. 2 software was used to measure and calculate of the bioactivity activity studies. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). StakMax Microplate Handling Stacker. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. SPECTRAmax® 190. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. 06 ng/mL; FSH: 0. 2 - 3. 5% phosphoric acid) in microplates at room temperature for 10 min. SpectraMax® Microplate Readers and SoftMax® Pro Software. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). The levels of rheumatoid factor (RF) antibodies were measured at the Clinical Immunology Laboratory of the Sahlgrenska University Hospital or Leiden Clinical Immunology Laboratory. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. AoSMCs were seeded into 6-well culture plates. Unit powers. 312 gain calibration failed. 0200-6117. 0. The reaction mixture was incubated for 5 min. Wavelength Selection. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. Awareness Technology, Inc. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. Call 1-800-745-2710. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. G. 1 mL of acetylacetone. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The reaction mixture was incubated for 5 min. See More Microplate Reader / Microtiter Plate Reader. Quick View. Percent viability was calculated by comparison with the control group. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. SpectraMax Plus 384 measuring instruments pdf manual download. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. ). plates can be used in the SPECTRAmax PLUS 384. Upgradeable modules are also available including western blot, cell imaging, and fast. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. , Fremont, CA, USA). All strains were tested three times and the results averaged. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 30. or microplate reader (e. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Each experiment was performed in duplicate. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. 81. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. Bioz Stars score: 86/100, based on 1 PubMed citations. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. well, and strip-well microplates. , Sunnyvale, CA). Alkaline phosphatase (ALP) activity. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Near bottom. 1x10 5 transduced, GFP + SK-NEP-1. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. 340 nm / 70 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Service Contracts, Preventative Maintenance and Performance. Wavelength Range. 305 reference level saturation. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Dissolve 13. Near bottom. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. SpectraMax 190 reads 96-well and strip-well plates. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Microscopy. Male 8 pin mini Din to Female 9D. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 5), L-ascorbic acid (25. 2. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. A. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). 2. 1. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. The plates were incubated for 4 additional hours at 37°C. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 1 mg mL −1 CDDP loaded A-CHAM, B-CHAM, C-CHAM or D-CHAM. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. (Shiga, Japan) and kept for 30 min in the dark. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. Description 1. Nitric Oxide Assay. View the Molecular Devices website. #N207) LAL Reagent Water (Lonza cat. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). application or budget. ) at 490 nm. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Microplate Readers. 2. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. These isotypes cause antigen cross-linking by multimeric binding and self. reader (SpectraMax 340; Molecular devices, LLc). The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. 6. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Each experiment was performed in duplicate and repeated at. No power cord or accessories included. Six wells were assayed for each set of conditions and stadard deviations. Clotting times (mean ± SD) for rSimukunin and rSV. The sensitivities of the hormones are Prolactin: 0. Other technologies such as imaging. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Assay setup for a 384-well low-volume plate. 0 2. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. . The plates were incubated for 4 additional hours at 37°C. 5% phosphoric acid) in microplates at room temperature for 10 min. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. ATI/240, 250. Call 1-800-745-2710. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Samples with OD values of less than 0. 5. Thin layer chromatography (TLC) was. 313 reference gain check fail. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Data were expressed as the mean LPS-treated cells (100%) vs. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. edu Contact Phone: 216-368-8962. D. Wells were washed three times. Examples: “Press carefully to engage,” “Do not press down. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. D2. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Read one sample or up to 384. chen@case. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Customer Transformation Hub. Streamline GLP/GMP compliance. Each experiment was performed in duplicate and repeated at. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). 7 macrophages were obtained from ATCC (Manassas, VA). (1988) and recorded at 412 nm. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Money Saved: $340. ). SpectraMax 340 from Molecular Devices). Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Assay buffer was 50 m M Hepes, pH 7. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. Novak, Nathan J. 2. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 5 mL of 1 M acetate buffer (pH 4. 7. The values were corrected for nonspecific background by subtracting the OD 650. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. Immunofluorescence and Aggregation Analysis. chen@case. Heme was preincubated for 5 min with A when added before the metals. Page: 1/6. Although all samples initially were analyzed at the same volume, the volume of sample. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 4) was followed according to Vanacker et al. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. Please see photos. SpectraMAX 340. SpectraMax ® Plus 384 optics. 6 (h) x 22. Driessen*,MaaikeA. Features showcased by this model include. Or scan up to 384 wells at any time to determine the best assay conditions. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 4; 100 m M NaCl, 0. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The growth. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. . 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). 00. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Operators can conveniently select up to 6 wavelengths at a time for. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. chen@case. 024). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Incubation with TMB substrate was done for 18 min. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. 30. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. Integration delay. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). SpectraMax Multi-Mode Microplate Readers. 3. 2. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Service parts for these models are not being produced and are no longer in stock.